Photosynthesis
By the end of this chapter you will be able to
- Write the word equation and the balanced symbol equation for photosynthesis
- Name the products of photosynthesis and say where each is used
- Identify the limiting factor on a graph, and explain the effect of each factor
- Carry out and interpret the leaf-disc practical, and calculate a rate
- [H] Use the inverse square law to compare light intensities at different distances
You should already know
- Chloroplasts and the structure of leaves (Chapter 8)
- Enzymes and temperature (Chapter 5)
- Aerobic respiration (Chapter 10 links back)
Where this leads
- Aerobic and anaerobic respiration (Chapter 10)
- Carbon cycle (Chapter 17)
- Nitrate and proteins (Chapter 8)
GCSE Biology. Content follows the GCSE (9-1) subject content for England (Edexcel and AQA). Content references: Write the word and symbol equations for photosynthesis, Describe chloroplasts and chlorophyll, and the products of photosynthesis, Explain limiting factors (light, carbon dioxide, temperature) and interpret graphs of them, Investigate the effect of light intensity on the rate of photosynthesis, using leaf discs, Describe the uses of glucose made by photosynthesis, and how greenhouses control the factors, [H] Apply the inverse square law to light intensity.
Exercise P Do you remember?
Method. Divide 1000 by 20.
- Divide. 1000 / 20 = 50.1000 / 20 = 50
- Write the answer. 50
Check. 50 x 20 = 1000. ✓
Method. Recall the organelle.
- Recall. Chlorophyll is found in chloroplasts.found in chloroplasts
- Write the answer. chloroplast
Check. Mitochondria do not contain chlorophyll. ✓
Method. Recall the gas used.
- Recall. Photosynthesis takes in carbon dioxide from the air.takes in carbon dioxide
- Write the answer. carbon dioxide
Check. Oxygen is released. ✓
Method. Recall the mineral for proteins.
- Recall. Nitrate is needed to make amino acids and proteins.amino acids and proteins
- Write the answer. nitrate
Check. Magnesium is needed for chlorophyll. ✓
Method. Multiply four twos.
- Multiply. 2 x 2 = 4, 4 x 2 = 8, 8 x 2 = 16.2 x 2 x 2 x 2 = 16
- Write the answer. 16
Check. 16 is 2 to the power 4. ✓
The photosynthesis equation and where it happens
Photosynthesis is the process by which plants make glucose from carbon dioxide and water, using light energy. It releases oxygen as a by-product. It happens in chloroplasts, which contain the green pigment chlorophyll. Chlorophyll absorbs the light energy needed for the reaction.
Word equation: carbon dioxide + water -> glucose + oxygen (light energy and chlorophyll are needed).
Symbol equation: 6CO2 + 6H2O -> C6H12O6 + 6O2.
Key ideas
- Photosynthesis makes glucose and oxygen from carbon dioxide and water, using light.
- It takes place in chloroplasts, which contain chlorophyll.
- Chlorophyll absorbs light energy.
- The symbol equation has 6 carbon dioxide and 6 water molecules on the left.
Method. Name the reactants and the products.
- Reactants. The reactants are carbon dioxide and water.carbon dioxide and water
- Products. The products are glucose and oxygen.glucose and oxygen
- Write it. Carbon dioxide + water gives glucose + oxygen, in light and with chlorophyll.in light
- Write the answer. carbon dioxide + water -> glucose + oxygen
Check. The equation balances in atoms with six of each. ✓
Method. Recall the by-product.
- Recall. Photosynthesis releases oxygen into the air.releases oxygen
- Name it. So the by-product is oxygen.oxygen
- Write the answer. oxygen
Check. Carbon dioxide is taken in, not released. ✓
Method. Recall the green pigment.
- Recall. The green pigment in chloroplasts absorbs light.absorbs light
- Name it. This pigment is chlorophyll.chlorophyll
- Write the answer. chlorophyll
Check. Chlorophyll is green because it reflects green light. ✓
Watch out
Watch out: photosynthesis needs light, but it is not the same as respiration. Photosynthesis makes glucose; respiration releases energy from glucose.
Exam tip
Exam tip. In a symbol equation, count the atoms on each side. A balanced equation has the same number of each atom on each side.
Try it (with your teacher). With your teacher, look at a leaf under a lamp for a few minutes, then test it with iodine solution after a day, or use a model of a chloroplast. Describe what chlorophyll does, in one sentence.
Exercise 9A The photosynthesis equation and where it happens
Method. Recall the starting substances.
- Recall. Photosynthesis uses carbon dioxide and water as reactants.carbon dioxide and water
- Write the answer. carbon dioxide and water
Check. Light is an energy source, not a reactant. ✓
Method. Recall the product.
- Recall. Photosynthesis makes glucose.makes glucose
- Write the answer. glucose
Check. Glucose is used for respiration and other things. ✓
Method. Recall the gas released.
- Recall. Oxygen is released as a by-product of photosynthesis.released as a by-product
- Write the answer. oxygen
Check. Carbon dioxide is absorbed. ✓
Method. Recall the organelle.
- Recall. Photosynthesis happens in chloroplasts.in chloroplasts
- Write the answer. chloroplast
Check. Chloroplasts contain chlorophyll. ✓
Method. Put the reactants on the left and the products on the right.
- Left. The reactants are carbon dioxide and water.carbon dioxide + water
- Right. The products are glucose and oxygen.glucose + oxygen
- Write the answer. carbon dioxide + water -> glucose + oxygen
Check. Light energy is written above the arrow. ✓
Method. Read the symbol equation.
- Read. The symbol equation has 6CO2 on the left for one C6H12O6 on the right.6CO2 for one glucose
- Write the answer. 6
Check. Six carbon atoms are in one glucose. ✓
Method. Read the symbol equation.
- Read. The symbol equation gives 6O2 on the right.6O2
- Write the answer. 6
Check. Each glucose needs six oxygen molecules to be released. ✓
Method. Recall the pigment.
- Recall. Chlorophyll absorbs light energy for photosynthesis.absorbs light energy
- Write the answer. chlorophyll
Check. Chlorophyll is in the chloroplasts. ✓
Method. Think about what a reactant is.
- Think. A reactant is a substance that is used up in the chemical reaction.used up
- Decide. Light is an energy source, not a substance that is used up.energy source
- Write the answer. So light is not a reactant; the answer is no.no
- Write the answer. no
Check. The light is written above the arrow. ✓
Method. Think about the cells with the most chloroplasts.
- Think. The palisade cells near the top have many chloroplasts.many chloroplasts
- Conclude. So most photosynthesis happens in the palisade layer.palisade layer
- Write the answer. palisade
Check. The lower epidermis has few chloroplasts. ✓
Method. Think about the energy.
- Think. Making glucose needs energy, which comes from light.comes from light
- Conclude. Chlorophyll absorbs the light energy and uses it to make glucose.absorbs the light energy
- Write the answer. Accept any sentence that says the light energy is absorbed by chlorophyll and used to make glucose.
Check. Light is the energy source. ✓
Method. Link the position to the light.
- Light. The upper surface receives the most light.most light
- Position. Chloroplasts near the top can absorb that light efficiently.absorb that light
- Conclude. So the arrangement makes the most of the light energy.most of the light
- Write the answer. Accept any answer that says the top gets the most light, so the chloroplasts there make the most glucose.
Check. Position fits the function. ✓
Limiting factors
A limiting factor is the factor in shortest supply, which stops the rate of photosynthesis from increasing further. Three main factors can be limiting: the light intensity, the carbon dioxide concentration and the temperature.
When light is the limiting factor, the rate rises as the light increases, then levels off once something else limits it. When carbon dioxide is limiting, adding more carbon dioxide raises the rate. Temperature affects the enzymes that control photosynthesis; the rate rises with temperature up to an optimum, then falls sharply when the enzymes denature.
Key ideas
- A limiting factor is the factor in shortest supply.
- Light, carbon dioxide and temperature can each limit the rate.
- A graph levels off when a different factor becomes limiting.
- Temperature above the optimum denatures the enzymes.
Method. Find the first point where the line becomes flat.
- Look. The rate is 10 at light 3, and it stays at 10 at 4 and 5.stays at 10
- Read. So the rate first levels off at light intensity 3.3
- Write the answer. 3
Check. The rate does not change after 3. ✓
Method. Read the point at 4.
- Read. At a carbon dioxide level of 4 the rate is 10.10
- Compare. The rate has levelled off, so another factor now limits it.levelled off
- Write the answer. 10
Check. It is the same as at level 3. ✓
Method. Think about what happens above the optimum.
- Look. At 50 C the rate has fallen, because the enzymes have denatured.denatured
- Name it. The limiting factor is the temperature, which has denatured the enzymes.temperature
- Write the answer. temperature
Check. Temperature can be limiting at both low and high values. ✓
Watch out
Watch out: a graph levels off because a different factor is limiting, not because the factor you changed has stopped working. Name the factor that is limiting.
Exam tip
Exam tip. When you name a limiting factor from a graph, quote the point where the line levels off, and say which factor is now limiting.
Try it (with your teacher). With your teacher, sketch the light graph on squared paper. Mark the point where the line levels off, and write the limiting factor at that point.
Exercise 9B Limiting factors
Method. Recall the factors.
- Recall. Light, carbon dioxide and temperature can each limit photosynthesis.light, carbon dioxide and temperature
- Write the answer. light intensity, carbon dioxide concentration and temperature
Check. Water is also needed, but is rarely the limit in a leaf. ✓
Method. Recall the definition.
- Recall. A limiting factor is the factor in shortest supply, which stops the rate increasing.in shortest supply
- Write the answer. the factor in shortest supply
Check. Only one factor is limiting at a time. ✓
Method. Read the point at 2.
- Read. At light intensity 2 the rate is 8.8
- Write the answer. 8
Check. The rate rises from 4 to 8 between light 1 and light 2. ✓
Method. Read the point at 4.
- Read. At light intensity 4 the rate is 10.10
- Write the answer. 10
Check. The rate is level at 10 from light 3. ✓
Method. Find the highest point.
- Look. The highest value on the graph is 12.12
- Read. This is at 40 degrees C.40
- Write the answer. 40
Check. The rate falls to 4 at 50 degrees C. ✓
Method. Think about why the rate has levelled off.
- Think. Carbon dioxide is high enough now, so it no longer limits the rate.no longer limits
- Conclude. Another factor, such as light or temperature, must now be limiting.another factor
- Write the answer. Accept light or temperature.
Check. The graph alone cannot say which one is limiting. ✓
Method. Think about the light.
- Think. Light is the limiting factor below 3, so more light means more energy is absorbed.more energy absorbed
- Conclude. So more glucose is made, and the rate rises.rate rises
- Write the answer. Accept any sentence that says more light energy is absorbed by chlorophyll, so more glucose is made.
Check. The rise is due to light, not to carbon dioxide. ✓
Method. Think about the enzymes.
- Think. Photosynthesis uses enzymes, which are proteins.uses enzymes
- Conclude. At high temperature they denature, so the rate falls.denature
- Write the answer. Accept any sentence that says the enzymes denature, so the rate of photosynthesis falls.
Check. Denaturing is permanent. ✓
Method. Check the light graph.
- Check. The graph levels off, so light stops increasing the rate at high intensity.levels off
- Decide. So the statement is not always true.not always true
- Write the answer. The answer is no.no
- Write the answer. no
Check. Another factor limits the rate at high light. ✓
Method. Think about each limiting factor.
- Each factor. Light, carbon dioxide and temperature can each be the limiting factor.each be the limiting factor
- Conclude. So all three must be kept high for the rate to be high.all three
- Write the answer. Accept any answer that says each factor can be limiting, so all of them must be kept at a high level.
Check. Raising only one factor may not raise the rate. ✓
Required practical: light intensity and the rate of photosynthesis
In this practical the independent variable is the distance of the lamp from the leaf discs, which changes the light intensity. The dependent variable is the time for half the discs to float. The rate is calculated from the time: rate = 1000 / time (a convenient scale for this data). The control variables include the number of discs, the volume of solution, the temperature and the carbon dioxide level (the bicarbonate solution).
Light intensity falls as the lamp moves away, so a shorter time to float means a higher rate.
| Distance from lamp (cm) | Time to float (s) | Rate = 1000 / time |
|---|---|---|
| 10 | 50 | 20 |
| 20 | 100 | 10 |
| 30 | 200 | 5 |
| 40 | 500 | 2 |
Key ideas
- Independent variable: distance of the lamp (light intensity). Dependent variable: time to float.
- Rate = 1000 / time, so a shorter time gives a higher rate.
- Keep the number of discs, the volume and the temperature the same.
- Light intensity falls as the distance from the lamp increases.
Method. Divide 1000 by the time.
- Read. The time to float at 20 cm is 100 seconds.100 s
- Divide. Rate = 1000 / 100.1000 / 100 = 10
- Write the answer. 10
Check. 10 x 100 = 1000. ✓
Method. Divide the larger rate by the smaller.
- Read. The rate at 10 cm is 20, and at 40 cm it is 2.20 and 2
- Divide. Divide the larger rate by the smaller: 20 / 2 = 10.20 / 2 = 10
- Write the answer. 10
Check. 2 x 10 = 20. ✓
Method. Find the variable changed on purpose.
- Find it. The distance of the lamp is changed on purpose.changed on purpose
- Name it. So the independent variable is the distance from the lamp, which sets the light intensity.distance from the lamp
- Write the answer. distance from the lamp
Check. The time to float is the dependent variable. ✓
Watch out
Watch out: a shorter time gives a higher rate. Do not multiply the time by 1000, because the rate is 1000 divided by the time.
Exam tip
Safety. The lamp is set and checked by the teacher. Keep electrical equipment away from water, and handle the leaf discs and solutions with care.
Try it (with your teacher). With your teacher, set up leaf discs in sodium hydrogen carbonate solution at two lamp distances. Record the time for half the discs to float, and calculate the rate for each.
Exercise 9C Required practical: light intensity and the rate of photosynthesis
Method. Find what is measured.
- Find it. The time for the discs to float is measured.time to float
- Write the answer. time for half the discs to float
Check. The lamp distance is the independent variable. ✓
Method. Divide 1000 by the time.
- Divide. 1000 / 500 = 2.1000 / 500 = 2
- Write the answer. 2
Check. 2 x 500 = 1000. ✓
Method. Divide 1000 by the time.
- Divide. 1000 / 50 = 20.1000 / 50 = 20
- Write the answer. 20
Check. 20 x 50 = 1000. ✓
Method. Find the smallest rate.
- Find. The smallest rate is 2, at 40 cm.2 at 40 cm
- Name it. So the lowest rate is at 40 cm.40
- Write the answer. 40
Check. The discs take longest to float at 40 cm. ✓
Method. Recall the product of photosynthesis.
- Recall. Photosynthesis releases oxygen into the spaces in the leaf, which makes the discs float.releases oxygen
- Write the answer. oxygen
Check. The bubbles of oxygen lift the discs. ✓
Method. Think about the control variables.
- Think. The disc size and source are control variables.control variables
- Conclude. So only the light intensity changes between the tests.only the light
- Write the answer. Accept any sentence that says they are control variables, so only the light changes between tests.
Check. Different discs would give different results. ✓
Method. Calculate the rate, then compare.
- Calculate. 1000 / 200 = 5.1000 / 200 = 5
- Compare. The rate at 20 cm is 10, so 5 is lower.lower
- Write the answer. Accept rate 5, lower.
Check. The rate falls as the lamp moves away. ✓
Method. Read the table from the nearest to the furthest lamp.
- Read. The rate falls from 20 at 10 cm to 2 at 40 cm.20 to 2
- Describe. So the rate falls as the distance from the lamp increases.falls as the distance
- Write the answer. Accept any sentence that says the rate falls as the distance from the lamp increases.
Check. Light intensity falls as the distance increases. ✓
Method. Find a weakness and its fix.
- Weakness. Only one set of discs was used at each distance.one set of discs
- Fix. Repeating each distance and taking a mean makes the rate more reliable.more reliable
- Write the answer. Accept any sensible improvement, such as testing more distances or repeating each distance.
Check. A specific improvement answers a named weakness. ✓
Method. Link the light to oxygen to floating.
- Light. A closer lamp gives more light, so more light energy is absorbed.more light
- Oxygen. More photosynthesis releases more oxygen into the leaf.more oxygen
- Conclude. The bubbles of oxygen lift the discs sooner.lift the discs sooner
- Write the answer. Accept any answer that says more light is absorbed, so more oxygen is made and the discs float sooner.
Check. Floating is a measure of oxygen production. ✓
Uses of glucose and greenhouses
Glucose made by photosynthesis is used in several ways. It is used in respiration to release energy. It is converted to starch for storage. It is made into cellulose for cell walls. It is made into fats and oils for storage in seeds. With nitrate ions from the soil, it is used to make proteins.
A greenhouse can raise the rate of photosynthesis by adding carbon dioxide, providing extra light, and keeping the temperature at an optimum for the enzymes.
| Use of glucose | What happens to it |
|---|---|
| respiration | released as energy in the mitochondria |
| starch | stored in the leaves and roots |
| cellulose | made into cell walls for growth |
| fats and oils | made for storage in seeds |
| proteins | made using glucose plus nitrate ions |
Key ideas
- Glucose is used in respiration, stored as starch, made into cellulose, fats and oils, and proteins.
- Greenhouses can add carbon dioxide, extra light and heat.
- Raising the limiting factor raises the rate of photosynthesis.
- Glucose is also converted to starch for storage in leaves and roots.
Method. Recall the storage carbohydrate.
- Recall. Glucose is stored in plants as starch.stored as starch
- Name it. So the storage carbohydrate is starch.starch
- Write the answer. starch
Check. Cellulose is for cell walls, not for storage. ✓
Method. Recall the product.
- Recall. Glucose and nitrate ions are used together to make proteins.make proteins
- Name it. So the product is protein.protein
- Write the answer. protein
Check. Nitrate is the mineral from the soil. ✓
Method. Think about the limiting factor.
- Think. Carbon dioxide can be the limiting factor in a sealed greenhouse.limiting factor
- Conclude. Adding more lets the rate of photosynthesis increase.rate increase
- Write the answer. Adding carbon dioxide removes it as the limiting factor, so the rate of photosynthesis can increase.
Check. Greenhouses control several factors at once. ✓
Watch out
Watch out: adding more of one factor only helps if that factor is the limiting one. Above that point, the rate levels off.
Exam tip
Exam tip. When you explain a greenhouse measure, name the limiting factor it removes, and say how that raises the rate.
Try it (with your teacher). Write a list of the things a greenhouse controls, and the limiting factor each one controls. Check your list with your teacher.
Exercise 9D Uses of glucose and greenhouses
Method. Recall the process.
- Recall. Glucose is broken down in respiration to release energy.release energy
- Write the answer. respiration
Check. Respiration happens in mitochondria. ✓
Method. Recall the storage form.
- Recall. Glucose is stored as starch in leaves and roots.stored as starch
- Write the answer. starch
Check. Starch is made of many glucose units. ✓
Method. Recall the cell wall substance.
- Recall. Cellulose is made from glucose and forms plant cell walls.cell walls
- Write the answer. cellulose
Check. Cellulose is a strong fibre. ✓
Method. Recall the storage of fats and oils.
- Recall. Fats and oils are stored in seeds.stored in seeds
- Write the answer. seeds
Check. Seeds store energy for germination. ✓
Method. Recall the mineral.
- Recall. Nitrate ions are needed to make proteins from glucose.nitrate ions
- Write the answer. nitrate
Check. Magnesium is needed for chlorophyll. ✓
Method. Recall the factors.
- Recall. Greenhouses control carbon dioxide, light and temperature.carbon dioxide, light and temperature
- Choose one. Any one of these is a correct answer.any one
- Write the answer. Accept any one: carbon dioxide, light, or temperature.
Check. Each one can be limiting. ✓
Method. Recall why temperature matters.
- Recall. Photosynthesis uses enzymes, which work best at an optimum temperature.work best at an optimum
- Write the answer. enzymes work best
Check. Too hot and the enzymes denature. ✓
Method. Recall the energy source for respiration.
- Recall. Respiration breaks down glucose to release energy.breaks down glucose
- Write the answer. glucose
Check. Oxygen is used in respiration, not made for it. ✓
Method. Multiply the rate by the time.
- Multiply. 5 x 6 = 30.5 x 6 = 30
- Write the answer. 30
Check. 30 divided by 6 is 5. ✓
Method. Think about the limiting factor.
- Think. Only the limiting factor raises the rate when it is increased.limiting factor
- Conclude. If carbon dioxide is limiting, extra light will not help much.will not help
- Write the answer. Accept any sentence that says extra light does not raise the rate when another factor is the limit.
Check. Find the limit before adding a factor. ✓
Method. Think about what has changed.
- Limit. Once carbon dioxide is high enough, another factor must be limiting.another factor
- Check. The grower should check the light level and the temperature.check the light
- Write the answer. Accept any answer that says light or temperature is now limiting, so the grower should check those factors.
Check. The limit moves when one factor is raised. ✓
The inverse square law for light intensity [H]
[H] Higher tier only. This section is higher-tier content. It applies the inverse square law to the light from a point source, such as a lamp.
[H] For a point source of light, the intensity falls with the square of the distance. Doubling the distance cuts the intensity to a quarter, because the light spreads over four times the area. Tripling the distance cuts it to a ninth. This is the inverse square law: intensity is proportional to 1 / distance squared.
| Distance (cm) | Relative light intensity |
|---|---|
| 10 | 100 |
| 20 | 25 |
| 40 | 6.25 |
Key ideas
- [H] Intensity is proportional to 1 / distance squared.
- [H] Doubling the distance gives a quarter of the intensity.
- [H] Tripling the distance gives a ninth of the intensity.
- [H] Light intensity is the limiting factor for the rate at low light.
Method. Doubling the distance gives a quarter of the intensity.
- Double. The distance doubles from 10 cm to 20 cm.10 to 20
- Divide. The intensity becomes 100 / 4 = 25 units.100 / 4 = 25
- Write the answer. 25
Check. 25 x 4 = 100. ✓
Method. Tripling the distance gives a ninth of the intensity.
- Triple. The distance triples from 10 cm to 30 cm.10 to 30
- Divide. The intensity becomes 100 / 9, which is about 11.100 / 9 = 11.1
- Write the answer. 11
Check. 11 x 9 = 99, which is close to 100. ✓
Method. Think about how the light spreads.
- Think. The light spreads over a larger area as the distance increases.larger area
- Conclude. So the intensity falls with the square of the distance, not in direct proportion.square of the distance
- Write the answer. The light spreads over a larger area, so intensity falls with the square of the distance, not in direct proportion.
Check. Doubling distance does not halve the intensity. ✓
Watch out
Watch out: doubling the distance does not halve the intensity. It cuts it to a quarter, because the distance is squared.
Exam tip
Exam tip. [H] Use the square of the distance in the calculation: 2 squared is 4, and 3 squared is 9.
Try it (with your teacher). With your teacher, use a torch or a lamp and a ruler. Measure the spot of light on a card at 10 cm and 20 cm. Compare the brightness of the spots, and say which result matches the inverse square law.
Exercise 9E [H] The inverse square law
Method. Recall the inverse square law.
- Recall. Intensity falls with the square of the distance.square of the distance
- Name it. So intensity is proportional to 1 / distance squared.1 / distance squared
- Write the answer. one divided by the distance squared
Check. Distance squared means distance times distance. ✓
Method. Apply the law.
- Square. Doubling the distance means the distance squared is 4 times greater.2 squared is 4
- Fraction. So the intensity is one quarter of the original.1/4
- Write the answer. 1/4
Check. 1 divided by 4 is one quarter. ✓
Method. Apply the law.
- Square. Tripling the distance means the distance squared is 9 times greater.3 squared is 9
- Fraction. So the intensity is one ninth of the original.1/9
- Write the answer. 1/9
Check. 9 x 1/9 = 1. ✓
Method. Quadrupling the distance gives 1/16 of the intensity.
- Square. The distance is 4 times as large, so its square is 16 times as large.4 squared is 16
- Divide. The intensity is 100 / 16 = 6.25 units.100 / 16 = 6.25
- Write the answer. 6.25
Check. 6.25 x 16 = 100. ✓
Method. Doubling the distance gives a quarter.
- Double. The distance doubles from 20 cm to 40 cm.20 to 40
- Divide. The intensity becomes 25 / 4 = 6.25 units.25 / 4 = 6.25
- Write the answer. 6.25
Check. 6.25 x 4 = 25. ✓
Method. Think about the area.
- Think. Doubling the distance spreads the light over four times the area.four times the area
- Conclude. So the intensity falls to a quarter, not a half.to a quarter
- Write the answer. Accept any sentence that says the light spreads over four times the area, so the intensity falls to a quarter.
Check. Area scales with distance squared. ✓
Method. Check the law against the claim.
- Check. The law says the intensity falls to a quarter, not a half.a quarter
- Correct. So the intensity at 20 cm is 100 / 4 = 25 units.25 units
- Decide. So the pupil is not correct.not correct
- Write the answer. Accept no, 25 units.
Check. Doubling distance quarters the light. ✓
Method. Compare the light at the two distances.
- Light. At 40 cm the distance is 4 times, so the light is 1/16 of that at 10 cm.1/16
- Effect. Less light means less photosynthesis, so less oxygen is made.less oxygen
- Conclude. So the discs take much longer to float at 40 cm.much longer
- Write the answer. Accept any answer that says the light at 40 cm is 1/16 of the light at 10 cm, so less photosynthesis happens and the discs float more slowly.
Check. The inverse square law is a strong effect. ✓
Chapter summary
| Skill | Method |
|---|---|
| Equation | Carbon dioxide + water -> glucose + oxygen (light and chlorophyll); 6CO2 + 6H2O -> C6H12O6 + 6O2. |
| Chloroplast | Contains chlorophyll, which absorbs light energy for photosynthesis. |
| Limiting factors | Light, carbon dioxide and temperature; the rate levels off when another factor limits it. |
| Leaf-disc practical | Rate = 1000 / time to float; a closer lamp gives more light and a faster rate. |
| Glucose uses | Respiration, starch storage, cellulose for cell walls, fats and oils, and proteins with nitrate. |
| Greenhouses | Control carbon dioxide, light and temperature to remove the limiting factor. |
| [H] Inverse square | Intensity is proportional to 1 / distance squared; doubling the distance gives a quarter. |
Can you…?
- I can write the word and symbol equations for photosynthesis
- I can name the products of photosynthesis and say where they are used
- I can identify the limiting factor on a graph and explain the effect of each factor
- I can carry out the leaf-disc practical and calculate a rate
- I can explain how greenhouses control the factors that limit photosynthesis
- [H] I can apply the inverse square law to light intensity
Key terms
- photosynthesis
- the process by which plants make glucose from carbon dioxide and water, using light
- chloroplast
- the organelle where photosynthesis happens, containing chlorophyll
- chlorophyll
- the green pigment that absorbs light energy
- limiting factor
- the factor in shortest supply, which stops the rate increasing further
- optimum
- the value at which an enzyme works best
- starch
- the storage carbohydrate that plants make from glucose
- cellulose
- a strong carbohydrate that forms plant cell walls
- [H] inverse square law
- intensity is proportional to 1 divided by the distance squared
Exercise 9Z Check-up
Method. Recall the reactants and products.
- Recall. The reactants are carbon dioxide and water; the products are glucose and oxygen.reactants and products
- Write the answer. carbon dioxide + water -> glucose + oxygen
Check. Light and chlorophyll are needed. ✓
Method. Recall the organelle.
- Recall. Photosynthesis happens in chloroplasts.in chloroplasts
- Write the answer. chloroplast
Check. Chloroplasts contain chlorophyll. ✓
Method. Recall the pigment.
- Recall. Chlorophyll absorbs light energy.absorbs light energy
- Write the answer. chlorophyll
Check. Chlorophyll is in chloroplasts. ✓
Method. Recall the by-product.
- Recall. Oxygen is released by photosynthesis.released by photosynthesis
- Write the answer. oxygen
Check. Carbon dioxide is taken in. ✓
Method. Think about what has levelled off.
- Think. Once carbon dioxide is high, another factor such as light limits the rate.another factor
- Conclude. In the light graph, light is the limit after 3.light
- Write the answer. Accept light.
Check. The limit depends on the other factors. ✓
Method. Read the point at 1.
- Read. At light intensity 1 the rate is 4.4
- Write the answer. 4
Check. The rate rises from 0 to 4. ✓
Method. Divide 1000 by the time.
- Divide. 1000 / 100 = 10.1000 / 100 = 10
- Write the answer. 10
Check. 10 x 100 = 1000. ✓
Method. Recall the storage form.
- Recall. Plants store glucose as starch.store glucose as starch
- Write the answer. starch
Check. Cellulose is for the cell walls. ✓
Method. Recall the mineral.
- Recall. Nitrate ions are needed to make proteins.nitrate ions
- Write the answer. nitrate
Check. Protein needs nitrogen from nitrate. ✓
Method. Think about the limiting factor.
- Think. The limiting factor is the one in shortest supply.shortest supply
- Conclude. When light is no longer short, another factor limits the rate.another factor
- Write the answer. Accept any sentence that says another factor, such as carbon dioxide or temperature, is now the limiting factor.
Check. The limit moves to the next factor. ✓
Total 22 marks. Allow about 22 minutes.
Exercise 9X Exam-style questions
Method. Recall the process.
- Recall. Plants make glucose by photosynthesis.photosynthesis
- Write the answer. photosynthesis
Check. Respiration releases energy from glucose. ✓
Method. Recall the products.
- Recall. The products are glucose and oxygen.glucose and oxygen
- Write the answer. glucose and oxygen
Check. Carbon dioxide and water are the reactants. ✓
Method. Think about what is still changing.
- Think. Light and carbon dioxide are both high, so neither one is limiting now.neither one is limiting
- Name. So another factor, such as temperature, must be limiting the rate.temperature
- Write the answer. Accept temperature.
Check. The graph shows which factor is limiting. ✓
Method. Balance the carbon, hydrogen and oxygen atoms.
- Carbon. Six carbon atoms are needed on each side, from 6CO2 and C6H12O6.6 carbon atoms
- Balance. So the equation is 6CO2 + 6H2O -> C6H12O6 + 6O2.6CO2 + 6H2O -> C6H12O6 + 6O2
- Write the answer. 6CO2 + 6H2O -> C6H12O6 + 6O2
Check. Count each atom on both sides. ✓
Method. Link the carbon dioxide to the rate.
- Limit. Carbon dioxide may be the limiting factor in a sealed greenhouse.limiting factor
- Effect. Adding more raises the rate until another factor becomes the limit.another factor
- Write the answer. Accept any answer that says carbon dioxide may be limiting, and adding it raises the rate until another factor limits.
Check. The rate rises only while carbon dioxide is limiting. ✓
Method. Give three uses.
- Use 1. Glucose is used in respiration to release energy.respiration
- Use 2. Glucose is stored as starch, or made into fats and oils.stored as starch
- Use 3. Glucose is made into cellulose for cell walls, or into proteins with nitrate.cellulose
- Write the answer. Accept any three of: respiration to release energy; stored as starch; made into cellulose for cell walls; made into fats and oils; made into proteins with nitrate.
Check. Each use is a different route for the glucose. ✓
Method. Calculate each rate, then compare.
- Rate 1. 1000 / 50 = 20.1000 / 50 = 20
- Rate 2. 1000 / 500 = 2.1000 / 500 = 2
- Compare. 20 / 2 = 10, so the rate at 10 cm is 10 times faster.10 times faster
- Write the answer. Accept 20, 2, and 10 times faster.
Check. 2 x 10 = 20. ✓
Method. Describe the effect on the enzymes.
- Enzymes. Photosynthesis uses enzymes, which are proteins.uses enzymes
- Denature. Above the optimum they denature, so their active sites change shape.active sites change
- Conclude. So the substrate no longer fits and the rate falls.rate falls
- Write the answer. Accept any answer that says the enzymes denature at high temperature, so the active site changes shape and the rate falls.
Check. Denaturing is permanent. ✓
Method. Describe the rise.
- Rise. The rate rises from 0 to 10 as the light intensity rises from 0 to 3.rises from 0 to 10
- Conclude. So light is the limiting factor at low intensities.limiting factor
- Write the answer. Accept any answer that says the rate rises as light rises from 0 to 3, showing light is limiting.
Check. The rate levels off after 3. ✓
Method. Apply the inverse square law.
- Distance. The distance is three times as far, so the square is 9 times greater.3 squared is 9
- Intensity. The intensity is 100 / 9 = 11.1, about 11 units.100 / 9 = 11
- Explain. Light spreads over an area that grows with the distance squared.area
- Conclude. So the light falls very quickly as the lamp moves away.falls very quickly
- Write the answer. Accept 11 and an answer that says the intensity falls with the square of the distance.
Check. Check the calculation: 11 x 9 is about 100. ✓